Basicmedical Key

To quantify the changes in drug concentration that mark the different phases of the PK profile of a drug, scientists commonly calculate a parameter referred to as the half-life or…

The binary complex ES is commonly referred to as the ES complex, the initial encounter complex, or the Michaelis complex. As described above, formation of the ES complex represents a…

3.1 Enzyme–Inhibitor Binding Equilibria As we have seen before, the enzymatic reaction begins with the reversible binding of substrate (S) to the free enzyme (E) to form the ES complex,…

5.1 Concentration–Response Plots and IC50 Determination In Chapter 4 we briefly introduced the term IC50. Here we will describe its determination and meaning in greater detail. For any enzyme inhibitor…

The second type of system is termed an open system. This case is more reflective of how drugs and targets encounter one another in vivo. Here one of the components…

Figure 9.1 Typical enzyme reaction progress curve in the presence of an irreversible enzyme inactivator, highlighting the initial velocity region (vi) and the fact that the terminal velocity (vs) is…

4.1.2 Total, Background, and Specific Signal In the preceding section we defined the total signal and background signal as they relate to construction of a calibration or standard curve for…

Dehydrogenase Lovastatin and other statins HMG-CoA reductase Cholesterol lowering Methotrexate Dihydrofolate reductase Cancer, immunosuppression Nitecapone Catechol-O-methyltransferase Parkinson’s disease Norfloxacin DNA gyrase Urinary tract infections Omeprazole H+, K+ ATPase Peptic ulcers…