12 High-performance liquid chromatography Keypoints Introduction Instrumentation Stationary and mobile phases Structural factors which govern rate of elution of compounds from HPLC columns Elution of neutral compounds Control of elution…
2 Physical and chemical properties of drug molecules Introduction Calculation of pH value of aqueous solutions of strong and weak acids and bases Dissociation of water Strong acids and bases…
9 Mass spectrometry Keypoints Introduction Ion generation Electrospray ionisation (ESI) Atmospheric pressure chemical ionisation (APCI) Electron impact ionisation (EI) Matrix assisted laser desorption ionisation (MALDI) Other ionisation methods Ion separation…
8 Nuclear magnetic resonance spectroscopy Keypoints Introduction Instrumentation Proton (1H) NMR Chemical shifts Integration and equivalence Multiplicity and spin–spin coupling Splitting diagrams and spin systems Application of NMR to structure…
11 Gas chromatography Keypoints Introduction Instrumentation Syringes Injection systems GC oven Types of column Selectivity of liquid stationary phases Kovats indices and column polarity Examples of the separation of mixtures…
3 Titrimetric and chemical analysis methods Keypoints Introduction Instrumentation and reagents Glassware Primary standards and standard solutions Direct acid/base titrations in the aqueous phase Strong acid/strong base titrations Weak acid/strong…
5 Infrared spectrophotometry Keypoints Introduction Factors determining intensity and energy level of absorption in IR spectra Intensity of absorption Energy level of absorption Instrumentation Instrument calibration Sample preparation Application of…
4 Ultraviolet and visible spectroscopy Keypoints Introduction Factors governing absorption of radiation in the UV/visible region Beer–Lambert Law Instrumentation Diode array instruments Instrument calibration Calibration of absorbance scale Calibration of…
10 Chromatographic theory Introduction Void volume and capacity factor Calculation of column efficiency Origins of band broadening in HPLC Van Deemter equation in liquid chromatography Van Deemter equation in gas…
14 High-performance capillary electrophoresis Keypoints Introduction Electrophoresis EOF Migration in CE Instrumentation Control of separation Migration time Dispersion Applications of CE in pharmaceutical analysis Separation of atenolol and related impurities…
