Figure 17.3.1 Generation of a reporter construct to identify inhibitors of cell-to-cell signaling. (A) This panel represents the native promoter and gene that is regulated by CCS. (B)This panel demonstrates…
Figure 17.2.1 Pockets on the surface of a protein. For the color version of this figure, go to http://www.currentprotocols.com. Because many of these sites will be superfluous, it is important…
Key Points The β-lactam class of antibiotics includes penicillins, cephalosporins, carbapenems, and monobactams; many, particularly the newer examples, exhibit a broad spectrum of activity against both Gram negative and Gram…
Key Points Erythromycin, a commonly used macrolide antibiotic, has a broad spectrum of antibacterial activity which is similar to that of the penicillins, and so is frequently the agent of…
Key Points Tetracyclines are broad-spectrum agents with excellent bioavailability and activity against both Gram positive and Gram negative bacteria; resistance and issues of toxicity restrict their regular clinical use. New…
Key Points Bacteria can be classified according to their staining by the Gram stain (Gram positive, Gram negative, and mycobacteria) and their shape. Most bacterial (prokaryotic) cells differ from mammalian…
Table 3.1.1 Therapeutic indications for the sulfonamide antibiotics. Sulfonamide antibiotic Indications Co-trimoxazole (sulfamethoxazole and trimethoprim) Treatment prevention of Pneumocystis jirovecii pneumonia Treatment of toxoplasmosis Treatment of nocardiosis Sulfadiazine Prevention of…
Key Points The aminoglycoside antibiotics are potent bactericidal agents that are rapidly lethal to bacteria in a concentration-dependent manner; their therapeutic potential for widespread use is restricted by ototoxicity and…
Key Points Vancomycin is used for the treatment of meticillin-resistant Staphylococcus aureus (MRSA) infections. The glycopeptides bind to D-ala-D-ala and form a ‘protective layer’, thus preventing some of the steps…
