Fig. 7.1 Methods of protein purification. Various types of chromatography are shown in (a). Gel filtration chromatography (left panel) involves purification of proteins based on size. Small particles (red circles)…
Database Internet URL US National Library of Medicine—the National Library of Medicine (NLM), on the campus of National Institutes of Health (NIH) in Bethesda, Maryland, is the world’s largest medical…
Fig. 9.1 Argon laser with 488 nm wavelength. Photon release of light with characteristic wavelength. FITC 530 nm; PE 578 nm; PE-Cy5 670 nm Clinical Application The continued interest in…
Nonmaleficence Do not harm yourself or other people Beneficence Help yourself and other people Autonomy Allow rational individuals to make free, informed choices Justice Treat people fairly; treat equals equally,…
Breeding Blood/tissue collection Death as an endpoint Behavioral studies Administration of experimental agents Major survival surgery Antibody production/collection (monoclonal) using the ascites method Use of hazardous agents Multiple major survivor…
Melina R. Kibbe and Scott A. LeMaire (eds.)Success in Academic SurgerySuccess in Academic Surgery: Basic Science201410.1007/978-1-4471-4736-7_12© Springer-Verlag London 2014 12. Use of Genetically Engineered Mice for Research Kerry-Ann McDonald1 and Allan Tsung2 (1) Department of Surgery, University…
Curious Principle Number One Everyone wants a mentor Curious Principle Number Two Everyone enjoys talking about their mentor (sometimes more than other people like listening to them talking about their…
Fig. 8.1 RT-PCR. Following RNA purification and construction of tagged cDNAs, PCR amplification is done with quantitative results available in real time. PCR cycles required to shift from an initial…
Intramural Extramural Start-up from your own institution Personal donations and philanthropy Pilot grant funding (departmental or institutional) Disease-oriented foundations Regional Veterans Integrated Service Network (VISN) pilot funding Department of Veterans…
