22 Transmission electron microscopy Anthony E. Woods, John W. Stirling The fundamental advantage of transmission electron microscopy (TEM) over conventional light microscopy is that the electron microscope has a resolution…
18 Immunohistochemical techniques Peter Jackson, David Blythe Introduction The introduction of prognostic and predictive markers in immunohistochemistry has made a tremendous beneficial impact on patient diagnosis and management. This has…
20 Immunohistochemistry quality control Tracy Sanderson, Gregory Zardin Introduction Since its introduction into routine histopathology in the 1980s, immunohistochemistry has become established as an integral part of the diagnostic process….
3 Light microscopy John D. Bancroft, Alton D. Floyd Light and its properties Visible light occupies a very narrow portion of the electromagnetic spectrum. The electromagnetic spectrum extends from radio…
4 Fixation of tissues Anthony Rhodes Introduction Appropriate fixation of tissues for histological examination is extremely important. Without attention to this process, the range of tests performed in a modern…
13 Pigments and minerals Guy E. Orchard Introduction In biology, pigments are defined as substances occurring in living matter that absorb visible light (electromagnetic energy within a narrow band that…
21 Molecular pathology Diane L. Sterchi, Caroline Astbury Introduction Molecular pathology seeks to apply gene expression against morphology and use gene expression analysis to validate large numbers of targets. A…
15 Microorganisms Jeanine H. Bartlett Introduction We have all heard the expression, ‘The world is getting smaller’. Nowhere is that statement truer than in the world of microorganisms. Microorganisms (also…
