Biofunctionalization of Metals with Polymers
Fig. 6.1 Immobilization of NH2–PEG–NH2 by electrodeposition. The terminated PEGs electrically migrate to and are immobilized on the Ti cathode (a). More terminated amines combine with Ti oxide via an…
GENERAL SURGERY
Monoclonal Antibody Production and Applications
S. N. Property Conventional antiserum Monoclonal antibody 1. Determinant Several Single 2. Specificity Variable with animal and bleed Standard Partial cross-reactions with common determinants Unexpected cross-reactions may occur Seldom too…
Edible Vaccines
The concept of edible vaccine got impetus after Arntzen and coworkers expressed HBsAg in tobacco. The first edible vaccine was produced in tobacco in 1990 in which 0.02 % recombinant protein…
Proteomics
Fig. 8.1 Information transfer in the central dogma of biology Proteins perform vital functions in the body such as: Catalyzing various biochemical reactions through enzymes. Acting as messengers through neurotransmitters. Acting…
Recent Advances in Stem Cell Research
Fig. 9.1 Symmetric and asymmetric divisions (modified from http://stemcells.nih.gov/info/scireport/chapter1.asp) 9.3.3 Ability to Give Rise to Specialized Cell The ability of stem cells to give rise to specialized cells is a crucial…
DNA Sequencing: Methods and Applications
Fig. 2.1 The reading pattern of autoradiogram 2.3.2 Sanger Method Frederick Sanger developed an alternative method, rather than using chemical cleavage reactions, Sanger opted for a method involving a third form…
Metagenomics: The Exploration of Unculturable Microbial World
Fig. 7.1 Steps in metagenomic analysis . 7.2 Steps in Metagenomic Analysis 7.2.1 DNA Isolation In general, metagenomic DNA isolation strategy should result in representation from a broad range of organisms….
DNA Microarray
Fig. 6.1 Hybridization of the target to the probe in DNA microarray (Source http://en.wikipedia.org/wiki/DNA_microarray) Fig. 6.2 The steps involved in a microarray experiment (Source http://en.wikipedia.org/wiki/DNA_microarray) 6.4 Types and Fabrication of DNA Microarrays…
Gene Therapy
Disease Gene therapy Severe combined immunodeficiency (SCID) Adenosine deaminase (ADA) Cystic fibrosis Cystic fibrosis transmembrane regulator (CFTR) Sickle cell anemia β-Globin Hemophilia Factor IX Thalassemia α or β–Globin Peripheral artery…
High Capacity Vectors
Vector Host Insert size Plasmid E.coli 5–25 kb λ phage E.coli 35–45 kb P1 phage E.coli 70–100 kb PACs E.coli 100–300kb BACs E.coli <300 kb YACs Saccharomyces cerevisae 200–2000kb Human…
