23 Quantitative data from microscopic specimens Alton D. Floyd Introduction The practice of histology and histopathology has traditionally relied upon the subjective interpretation of microscopic preparations by a highly trained…
10 The hematoxylins and eosin John D. Bancroft, Christopher Layton Introduction The hematoxylin and eosin stain (H&E) is the most widely used histological stain. Its popularity is based on its…
9 How histological stains work Richard W. Horobin Introduction All histological staining methods, from acid dyeing to silver impregnation, are based on the same physicochemical principles, as will be described…
7 Microtomy Paraffin and frozen Lena T. Spencer, John D. Bancroft Introduction The basic principles of microtomy are applicable to both paraffin and frozen sections. This chapter will discuss the…
2 Safety and ergonomics in the laboratory John D. Bancroft There are numerous workplace hazards in histology laboratories, and most countries have now passed regulations designed to improve this. These…
1 Managing the laboratory Louise Dunk Introduction Management is an integral aspect of the day-to-day life of the histopathology laboratory and is a major requirement of the accreditation process required…
Herpes Simplex Pathogenesis and Epidemiology Transmitted by direct contact. Invades skin locally producing skin vesicles by its cytolytic activity. Remains latent in the sensory ganglia. Reactivation is triggered by physical…
Herpesviruses are enveloped, double-stranded DNA viruses (120–240 kb) encoding for more than 35 proteins. After an acute infection, lifelong latency follows with the potential for relapse to occur later in…
Leptospira There are two main species: Leptospira interrogans, which contains all of the pathogenic strains, and L. biflexa, a non-pathogen. L. interrogans has more than 200 serovariants that may be…
Pseudomonas spp. Most Pseudomonas spp. are environmental organisms that can cause opportunistic infections in a healthcare environment. Pseudomonas Aeruginosa This organism is widespread in the environment, but rare in the…
