Practical Structured Illumination Microscopy
Fig. 1 Moiré fringes. (a) and (b) are two examples of fine patterns. When one is superimposed onto the other, a coarser beat pattern—moiré fringes—appears (c) In optics, reciprocal space…
Fig. 1 Moiré fringes. (a) and (b) are two examples of fine patterns. When one is superimposed onto the other, a coarser beat pattern—moiré fringes—appears (c) In optics, reciprocal space…
Fig. 1 Signals detected by ratio imaging and FilterFRET. (a–c) Spectra of a perfect FRET pair, i.e., there is no leak-through and cross-excitation between the fluorophores. (d–f) Spectra of real-world…
Here, I is the maximum intensity of the depletion beam, and the saturation intensity I S is the intensity required to reduce the fluorescence probability by half [4]. Thus, larger…
Fig. 1 Principles of 2PE microscopy. (a) Jablonski energy diagram showing the electron excitation process in single- (left ) and two-photon (right ) fluorescence microscopy. Single-photon excitation (left ) requires the absorption of…
Fig. 1 4Pi setup. (a) Scheme of the commercially available 4Pi setup (TCS 4Pi microscope, type A, Leica Microsystems, Mannheim, Germany). O1 and O2: objective lenses; S: sample; BS: beamsplitter;…
Fig. 1 Cartoon diagram of the photoactivatable Rac (PA-Rac). A LOV domain and its C-terminal Jα helix are fused to the N-terminus of a constitutively active mutant of Rac1 and…
Plate type Advantage Drawback Proposed application 96-Well plate Large number of cells per well, area ~30 mm2 Low number of wells Validation screen with preselected low number of targets 384-Well…
Figure 10.2 Normal distribution. Source: PDA TR-59.5 Table 10.1 Significance of Cp Figure 10.3 Using a histogram of a CQA to produce a normal distribution curve. Source: www.moorestream.com.7 Cp=Process Capability….
Figure 7.2 ICH Q9 approach. Source: ICH Q9.7 The risk referred to, in this case, is the risk to the patient. But this risk may come from many places. There…