Localizing Protein–Protein Interactions in Living Cells Using Fluorescence Lifetime Imaging Microscopy
(1) (2)As shown in Eq. 1, the efficiency of energy transfer (E) from the donor to the acceptor is dependent on the inverse of the sixth power of the distance…
Homo-FRET Imaging Highlights the Nanoscale Organization of Cell Surface Molecules
Fig. 1 Principle of homo-FRET process. (a) Angular orientation of donor (cyan) and acceptor (yellow-orange) transition dipoles are shown as θ D and θ A, respectively, where the intermolecular distance…
Three-Dimensional Photoactivated Localization Microscopy with Genetically Expressed Probes
Fig. 1 Concept behind PALM. When labeled with a fluorescent probe and viewed through a conventional microscope, high spatial frequency detail in an object (leftmost column) is obscured, producing a…
Live Spheroid Formation Recorded with Light Sheet-Based Fluorescence Microscopy
Fig. 1 Light sheet-based fluorescence microscopy (LSFM). (a) Setup of a single plane illumination microscope (aka SPIM) [5]). (b) Principles of LSFM imaging. Left : a single plane in the specimen…
Analysis of Biomolecular Dynamics by FRAP and Computer Simulation
Fig. 1 General scheme for Monte Carlo simulation of FRAP on nuclear proteins. Overview of th e COMPARTMENTS that can be defined in the script language. The nucleus is represented…
The Physical Basis of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications
Fig. 1 TIRF versus epifluorescence imaging. A fixed MDA-MB-231 breast cancer cell labeled for alpha-tubulin (a, b) and phospho-paxillin (c, d). TIRF imaging (a, c) results in a much higher…
Fluorescence Correlation Spectroscopy
Fig. 1 FCCS setup. Light from two laser beams is focused into the sample using a highly corrected objective lens with a large numerical aperture. Fluorescence is guided via a…
Direct Stochastic Optical Reconstruction Microscopy (dSTORM)
Fig. 1 Principle of single-molecule super-resolution microscopy. (a) Photoswitchable fluorophores are transferred from a fluorescent bright state into a nonfluorescent dark state by the irradiation of light. (b) By activating…
