Nonregenerative Tissues
Fig. 2.1 The epidermis is a regenerative tissue. Following controlled injury (stripping or blistering), which leaves the dermis intact (left), the epidermis recovers its structure completely at the site of…
Regeneration of a Peripheral Nerve
Fig. 6.1 (Top; a): Construction of the characteristic curve for a silicone tube used as a bridge for a gap of variable length in the mouse (left) and rat (right)…
The Irreversibility of Organ Injury
Fig. 1.1 Amputation of newt limbs leads to spontaneous regeneration. Limbs were amputated either below (left) or above the elbow (right) and were photographed at the indicated times while regenerating….
The Scaffold Regeneration Paradigm and Its Consequences
Fig. 10.1 A scaffold with regenerative activity was initially synthesized as a highly porous graft copolymer of type I collagen and chondroitin 6-sulfate (GAG). It was modified later by omitting…
Experimental Methods I: The Anatomically Well-Defined Wound
Fig. 3.1 Selection of an anatomically well-defined defect for study of induced skin regeneration. Top left: Incision through the entire dermis. Top right: The epidermis has been removed but the…
Transmission electron microscopy
22 Transmission electron microscopy Anthony E. Woods, John W. Stirling The fundamental advantage of transmission electron microscopy (TEM) over conventional light microscopy is that the electron microscope has a resolution…
Immunohistochemical techniques
18 Immunohistochemical techniques Peter Jackson, David Blythe Introduction The introduction of prognostic and predictive markers in immunohistochemistry has made a tremendous beneficial impact on patient diagnosis and management. This has…
Immunohistochemistry quality control
20 Immunohistochemistry quality control Tracy Sanderson, Gregory Zardin Introduction Since its introduction into routine histopathology in the 1980s, immunohistochemistry has become established as an integral part of the diagnostic process….
Light microscopy
3 Light microscopy John D. Bancroft, Alton D. Floyd Light and its properties Visible light occupies a very narrow portion of the electromagnetic spectrum. The electromagnetic spectrum extends from radio…
Fixation of tissues
4 Fixation of tissues Anthony Rhodes Introduction Appropriate fixation of tissues for histological examination is extremely important. Without attention to this process, the range of tests performed in a modern…
