2.4 Internal Standards 1. 1α,25-Dihydroxyvitamin D3 (26,26,26,27,27,27-d6), Medical Isotopes, Pelham, NH. 2. 1α,25-Dihydroxyvitamin D3 (26,26,26,27,27,27-d6), Medical Isotopes, Pelham, NH. 3. Preparation of individual standard stocks (20 μg/mL):…
Calibrator Tertiary combo standards (μL) Secondary combo standards (μL) Serum volume Final conc. (ng/dL) Blank 10 mL 1 10 μL qs to 10 mL 10 2 20 μL qs to…
Fig. 1 HPLC -ESI-MS/MS ion chromatogram of SAM (m/z 399.1 > 250.1), SAH (m/z 385.1 > 136.1), SAM-D3 (m/z 402.1 > 250.1) and SAH-D4 (m/z 389.1 > 138.1). Concentration of SAM and SAH is 95 nM and 41…
Analyte Stock solution [Expected] pH, Max UV wavelength ε max × 10−3 Adenine 30 mg in 100 mL Buffer A 2.2 mM pH 6–8, 260 nm 13.3 Xanthinea 12 mg in 1…
7 % BSA (mL) 1° Standard (μL) PHE/TYR 2° Standard (μL) PHE/TYR Calibrator (mg/dL) PHE/TYR 0.940 –/– 50/10 0.5/0.1 0.930 20/– –/50 2.0/0.5 0.850 50/– –/100 5.0/1.0 0.880 100/20 –/–…
Compound Concentration (nmol/mL) D9-l-Carnitine (C0) 0.760 D3-Acetycarnitine (C2) 0.190 D3-Propionylcanitine (C3) 0.038 D3-Butyrylcarnitine (C4) 0.038 D9-Isovalerylcarnitine (C5) 0.038 D3-Octanoylcarnitine (C8) 0.038 D9-Tetradecanoylcarnitine (C14) 0.038 D3-Hexadecanoylcarnitine (C16) 0.076 2. Quality…
Fig. 1 HPLC -ESI-MS/MS ion chromatogram of 5-MTHF 100 nM standard. [5-MTHF 1 (m/z 460.3 > 313.3), 5-MTHF 2 (m/z 460.3 > 194.5), and 5-MTHF-13C5 (m/z 465.3 > 313.3)] Fig. 2 HPLC -ESI-MS/MS ion chromatogram of…
Std level (μU/mL) Volume of 100 μU/mL mixed calibrator (μL) Volume of serum pool ( μL) 2.5 12.5 487.5 5 25 475 10 50 450 25 125 375 50 250 250…
To make: Use source solution Volume of source solution (mL) Volume of CLRW Metanephrine concentration (ng/mL)a Normetanephrine concentration (ng/mL)a Std 5 Spiked from Stock 160 μL QS to 100 mL…
Ion Mass (amu) Charge state Mass accuracy IGF-1 analyte 1093.5209 +7 10 ppm 1093.3475 +7 10 ppm 1093.6640 +7 10 ppm Rat IGF-1Ox IS 1098.9622 +7 10 ppm Compound parameters…
