Fig. 1 Product ion mass spectra of molecular ion [M + H]+ of the n-dibutyl esters of (a) MMA, (b) SA, and (c) mixture of MMA and SA, ratio of the concentrations…
(1) where [AngI]37 ° C represents the concentration of AngI after incubation at 37 °C, [AngI]blank represents the concentration of AngI after incubation on ice-water bath and Δt is the duration of…
Calibration std conc. (pg/mL) Add working calibration std Mix 1, 1 pg/μL (μL) Add working calibration std Mix 2, 10 pg/μL (μL) Add working calibration std Mix 3, 100 pg/μL…
500 μM succinylacetone (μL) Water (μL) Succinylacetone (nmol) Cal 1 0 1500 0 Cal 2 25 1475 12.5 Cal 3 50 1450 25 Cal 4 75 1425 37.5 Cal…
Elapsed time (min) % Mobile phase B Flow rate (mL/min) 0 30 1.0 0.900 80 1.0 0.901 92 1.0 1.150 92 1.0 1.160 95 1.0 1.170 95 1.7 1.460 95…
Calibrator Volume of stock standard (μL) Volume of ethanol (μL) Final concentration (μg/mL) Final dilution 6 200 – 6.0 – 5 200 200 3.0 1:2 4 333 667 2.0 1:3…
Calibrator concentration pg/mL Intermediate 50 ng/mL std (mL) Stripped serum (mL) 0 0 250 50 0.25 249.75 100 0.50 249.5 300 1.50 248.5 600 3.0 247.0 1000 5.0 245.0 2000…
To make Use source solution Volume of source solution Volume of 10 mM ammonium phosphate pH 6.5 (mL) Metanephrine concentration (pg/mL) Normetanephrine concentration (pg/mL) Std7 Working solution 100 μL QS…
Pre- Name Synonym Supp. Mol. wt. Acetoacetic Li salt Lithium acetoacetate Sigma 108.00 N– Acetylaspartic Aldrich 175.10 N– Acetyltyrosine Sigma 223.20 Aconitic cis-Aconitic Sigma 174.10 Adipic Sigma 146.14 Benzoic-Na salt …
2.4 Internal Standards 1. 1α,25-Dihydroxyvitamin D3 (26,26,26,27,27,27-d6), Medical Isotopes, Pelham, NH. 2. 1α,25-Dihydroxyvitamin D3 (26,26,26,27,27,27-d6), Medical Isotopes, Pelham, NH. 3. Preparation of individual standard stocks (20 μg/mL):…
