Fat Tissue Analysis in the Management of Patients with Systemic Amyloidosis
Year Reference Type of amyloid Controls Amyloid patients Sensitivity (%) Specificity (%) PPV (%) NPV (%) 1986 [15] A, L, TTR 12 12 83 100 100 86 1987 [16] A…
Amyloidoses of the Kidney, the Lower Urinary and Genital Tracts (Male and Female), and the Breast
Fig. 28.1 Renal amyloid deposits—Congo red stains viewed under polarized light. (a) Glomerular and extraglomerular vessels and interstitial amyloid. (b) Glomerular deposits. (c) Interstitial amyloid. (d) Amyloid in the medullary…
Classification of Amyloidosis by Mass Spectrometry-Based Proteomics
Shotgun proteomics Targeted proteomics Tries to identify every protein in a mixture Tries to identify specific protein targets Analogous to gene expression profiling Analogous to RTPCR or immunoassays Semiquantitative Quantitative…
Light/Heavy Chain Deposition Disease as a Systemic Disorder
Fig. 10.1 Nodular glomerulosclerosis secondary to κ-LCDD. (a) X500 and (b) X750. (a) Hematoxylin and eosin and (b) immunohistochemical stain for κ-light chains. (a) The glomerulus displays the typical nodularity—nodular…
Amyloidosis of the Lymph Nodes and the Spleen
Fig. 32.1 Gross appearances of lymph node involvement in AL amyloidosis. Cut surface of the lymph node involved by AL amyloidosis shows replacement of the parenchyma with a waxy appearing…
Amyloidosis of the Lung
Fig. 33.1 Systemic ATTR amyloidosis involving the lung. Eosinophilic amorphous material consistent with vascular amyloid deposition in the lung (a, H&E, arrow) stained with Congo red (b, arrow). Congo red…
Peripheral Nerve Amyloidosis
Fig. 31.1 Shown are the characteristic features of amyloid deposition in peripheral sural nerve biopsy tissue studied by light microscopy. Paraffin-stained sections show an acellular and homogenous proteinaceous material on…
Options for Amyloid Typing in Renal Pathology: The Advantages of Frozen Section Immunofluorescence and a Summary of General Recommendations Regarding Immunohistochemistry Methods
Fig. 20.1 A case of AL-lambda. (a) Stain for lambda light chain. Notice the high signal-to noise ratio. (b) Stain for kappa light chain. Although the exposure had to be…
Diagnosis of Minimal Amyloid Deposits by Congo Red Fluorescence and Amyloid Type-Specific Immunohistochemistry: A Review
Fig. 14.1 Diagnosis of amyloid in fixed tissues after CR staining, as viewed under three different types of illumination (“triple illumination”), in order to demonstrate the detection of amyloid and…
Typing of Amyloid for Routine Use on Formalin-Fixed Paraffin Sections of 626 Patients by Applying Amyloid type-Specific Immunohistochemistry: A Review
Amyloid class No. of patients % of total ALλ 271 43.3 ALκ 118 18.8 (AL, sum) (389) (62.1) ATTR 93 14.9 AA 80 12.8 AFib 14 2.2 Aβ2M 11 1.8…
