Flow Cytometry
Flow Cytometry Friederike Kreisel I. BASIC PRINCIPLE. Flow cytometry simultaneously measures and analyzes multiple physical and/or chemical characteristics of single particles, usually cells, as they flow in a fluid stream…
PATHOLOGY & LABORATORY MEDICINE
Immunofluorescence
Immunofluorescence Anne C. Lind Joseph P. Gaut Rosa M. Dávila I. INTRODUCTION. Immunofluorescence studies are used to support fixed tissue diagnoses and to provide additional diagnostic and prognostic information as…
Immunohistochemistry
Immunohistochemistry Peter A. Humphrey I. INTRODUCTION. Immunohistochemistry is one of the most powerful and widely used ancillary methods in surgical pathology. The technique makes it possible to simultaneously visualize cell…
Histology and Histochemical Stains
Histology and Histochemical Stains Kevin Selle I. RECEIPT, ACCESSIONING, AND GROSS DISSECTION. Most, if not all, biopsies and large tissue specimens are routed to the pathology laboratory. Under normal circumstances,…
Frozen Sections and Other Intraoperative Consultations
Frozen Sections and Other Intraoperative Consultations Michael E. Hull Peter A. Humphrey John D. Pfeifer I. INTRODUCTION. Intraoperative consultations fall into two general categories. Microscopic consultations, usually performed as frozen…
Metabolic Diseases of Bone
Metabolic Diseases of Bone Deborah Novack I. NORMAL ANATOMY. Because of its accessibility and composition of both cortical and trabecular (cancellous) bone, the iliac crest is the site of choice…
General Principles of Cytopathology
General Principles of Cytopathology Brian Collins I. INTRODUCTION. Cytopathology has developed into a discipline that examines cellular elements from throughout the body collected by a wide variety of methods and…
Joints and Synovium
Joints and Synovium Peter A. Humphrey I. NORMAL ANATOMY. Joints are composed of the ends of contiguous bones and the associated soft tissue elements, including cartilage, ligaments, tendons, and synovium….
Bone Neoplasms and Other Nonmetabolic Disorders
Bone Neoplasms and Other Nonmetabolic Disorders Omar Hameed Michael J. Klein I. NORMAL MICROSCOPIC ANATOMY. The bones are composed of compact bone, which is derived from intramembranous ossification, and coarse…
Retroperitoneum
Retroperitoneum Catalina Amador-Ortiz Louis P. Dehner John D. Pfeifer I. NORMAL ANATOMY. The retroperitoneal space (in some respects a virtual space) is located between the posterior parietal peritoneum and the…
