Immunofluorescence



Immunofluorescence


Anne C. Lind

Joseph P. Gaut

Rosa M. Dávila





  • I. INTRODUCTION. Immunofluorescence studies are used to support fixed tissue diagnoses and to provide additional diagnostic and prognostic information as it relates to autoimmune diseases, vesiculobullous diseases, transplantation, and glomerular disease. Immunofluorescence requires fresh tissue submitted in a preservative nonfixative transport medium such as Michel’s medium, or fresh frozen tissue; currently, immunofluorescence is not routinely performed on formalin-fixed paraffinembedded (FFPE) tissue. For fresh tissue, the transport medium should be held at room temperature; temperature extremes should be avoided. A specialized microscope and a room where the majority of ambient light can be extinguished are required for either direct or indirect immunofluorescence examination.


  • II. DIRECT IMMUNOFLUORESCENCE

Oct 20, 2016 | Posted by in PATHOLOGY & LABORATORY MEDICINE | Comments Off on Immunofluorescence
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