Selecting the Right Embryo in Mitochondrial Disorders
Fig. 17.1 PGD cycles of the respective carrier females, performed in our center. Each cluster of bars represents an embryo with its tested blastomeres. The red dotted line represents the…
Expanding PGD Applications to Nontraditional Genetic and Non-genetic Conditions
Disease # Patient # Cycle # Transfers # Embryo transferred Pregnancy Birth ATa 1 3 2 3 1 1 BCNS (gorlin) 5 6 5 9 3 3 Brain tumor 1…
SNP Array, qPCR, and Next-Generation Sequencing-Based Comprehensive Chromosome Screening
Fig. 14.1 SNP array-based single cell copy number analysis plots using three different WGA methodologies. The Sigma Aldrich GenomePlex WGA4 provided the most accurate copy number assignments when compared to…
Current and Future Preimplantation Genetic Screening (PGS) Technology: From Arrays to Next-Generation Sequencing
Fig. 13.1 Application of microarray technologies for PGS. Following biopsy, DNA is amplified using WGA, and then each biopsy sample is labeled with green fluorescent tags. Green biopsy sample is…
Controlled Ovarian Stimulation for Follicular Recruitment and Oocyte Recovery in IVF
Fig. 3.1 Schematic representation of pituitary suppression regimens in IVF. (a) Long GnRH agonist regimen. (b) Short GnRH agonist regimen. (c) GnRH antagonist regimen Although early studies suggested the agonist…
Comprehensive Chromosomal Screening from Polar Body Biopsy to Blastocyst Trophectoderm Sampling: Evidences and Considerations
Fig. 7.1 Evolution of chromosomal mosaicism along embryo preimplantation development. Chromosomal mosaicism is likely to reach its highest level along preimplantation development at the cleavage stage. Its origin is thought…
Utilisation of Transcriptome-Based Biomarkers for Single Embryo Transfer
Study Methodological approach Samples Observed outcome No. of patients included Proposed biomarkers McKenzie et al. [38] QPCR Whole cumulus complex Oocyte, embryo 8 patients GREM1, HAS2, PTGS2, PTX3, TNFAIP6 Zhang…
Efficiency of Polar Body Biopsy on Aneuploidy Screening by DNA Microarray for Single Euploid Embryo Transfer
Biopsy Clinical pregnancy rate per transfer Ongoing pregnancy rate per transfer Implantation rate per transfer References Polar bodya 33 % (8/24) 29 % (7/24) 26 % (10/39) [31] 35.2 %…
The Development of PGD
© Springer International Publishing Switzerland 2015E Scott Sills (ed.)Screening the Single Euploid Embryo10.1007/978-3-319-16892-0_1 1. The Development of PGD Joy D. A. Delhanty1 (1) UCL Centre for PGD, Institute for Women’s Health, University College London, London, WCIE 6HX,…
Prediction of Embryo Viability by Morphokinetic Evaluation to Facilitate Single Transfer
© Springer International Publishing Switzerland 2015E Scott Sills (ed.)Screening the Single Euploid Embryo10.1007/978-3-319-16892-0_10 10. Prediction of Embryo Viability by Morphokinetic Evaluation to Facilitate Single Transfer Aisling Ahlström1, Alison Campbell2, Hans Jakob Ingerslev3 and Kirstine Kirkegaard4 (1) Sahlsgrenska University Hospital,…
