Basicmedical Key

The results in Figure 4.1 can be displayed graphically by plotting the log concentration versus response (Figure 4.2). Figure 4.2 Log concentration–response curves for cholinesters. Questions 1. The responses are…

According to the Law of Mass Action, the value of the dissociation constant (Kd) could be derived as follows: (2.2) It was fully appreciated at the time that Kd is…

Particular care must be to ensure that all the units are the same. For example, if 10 μL of a 1 mg/mL solution is added to 20 mL organ bath,…

To count the number of live cells in a suspension, the following procedure is followed: The haemocytometer is cleaned with alcohol and dried using tissue paper and lens tissue. The…

where the molar extinction coefficient of TNB is 1.36 × 104 L/mol/cm. This is the absorbance of a 1 M solution in a 1 cm light path. The total volume…

Elevated Plus Maze (Neophobia) Test The test apparatus consists of two open arms (45 cm×15 cm) and two enclosed arms at 180° to each other. The entire maze was made…

Two designs of transducer are used, (1) isotonic, involving changes in length of the muscle and (2) isometric, where the length of the tissue is kept constant by an opposing…

Figure 6.2 Chart® record of antagonism of acetylcholine by d-tubocurarine in the presence of 1 μM serine in leech isolated dorsal muscle. The bath volume was 25 mL, and the…

It should be seen that salbutamol is about 100 times less potent than isoprenaline, suggesting that both drugs were acting on β1-adrenoceptors in the heart. At the doses selected, neither…