Techniques in neuropathology
17 Techniques in neuropathology J. Robin Highley, Nicky Sullivan Introduction Neuropathology has classically been seen as something of a dark art by general histologists because of the tradition of using…
Tissue processing
6 Tissue processing Lena T. Spencer, John D. Bancroft Incorporating Microarray Wanda Grace Jones Introduction After the removal of a tissue sample from the patient, a series of physical and…
Carbohydrates
12 Carbohydrates Christopher Layton, John D. Bancroft Introduction The word ‘carbohydrate’ is used to encompass a large group of compounds with the general formula Cn(H2O)n. While the role of carbohydrates…
Plastic embedding for light microscopy
8 Plastic embedding for light microscopy Neil M. Hand Introduction Paraffin wax is a suitable embedding medium for most tissues, combining adequacy of tissue support with ease of sectioning on…
Connective and mesenchymal tissues with their stains
11 Connective and mesenchymal tissues with their stains John D. Bancroft, Christopher Layton Introduction Connective tissue is one of the four tissue types found throughout the body. The term ‘connect’…
The gross room/surgical cut-up
5 The gross room/surgical cut-up S. Kim Suvarna, Christopher Layton Introduction The dissection and preparation of any specimen for histological/microscopic analysis involves more than simply the tissue processing and section…
Quantitative data from microscopic specimens
23 Quantitative data from microscopic specimens Alton D. Floyd Introduction The practice of histology and histopathology has traditionally relied upon the subjective interpretation of microscopic preparations by a highly trained…
The hematoxylins and eosin
10 The hematoxylins and eosin John D. Bancroft, Christopher Layton Introduction The hematoxylin and eosin stain (H&E) is the most widely used histological stain. Its popularity is based on its…
How histological stains work
9 How histological stains work Richard W. Horobin Introduction All histological staining methods, from acid dyeing to silver impregnation, are based on the same physicochemical principles, as will be described…
Microtomy: Paraffin and frozen
7 Microtomy Paraffin and frozen Lena T. Spencer, John D. Bancroft Introduction The basic principles of microtomy are applicable to both paraffin and frozen sections. This chapter will discuss the…
