Application of STED Microscopy to Cell Biology Questions
Here, I is the maximum intensity of the depletion beam, and the saturation intensity I S is the intensity required to reduce the fluorescence probability by half [4]. Thus, larger…
Two-Photon Excitation Microscopy and Its Applications in Neuroscience
Fig. 1 Principles of 2PE microscopy. (a) Jablonski energy diagram showing the electron excitation process in single- (left ) and two-photon (right ) fluorescence microscopy. Single-photon excitation (left ) requires the absorption of…
4Pi Microscopy of the Nuclear Pore Complex
Fig. 1 4Pi setup. (a) Scheme of the commercially available 4Pi setup (TCS 4Pi microscope, type A, Leica Microsystems, Mannheim, Germany). O1 and O2: objective lenses; S: sample; BS: beamsplitter;…
Optogenetics: Optical Control of a Photoactivatable Rac in Living Cells
Fig. 1 Cartoon diagram of the photoactivatable Rac (PA-Rac). A LOV domain and its C-terminal Jα helix are fused to the N-terminus of a constitutively active mutant of Rac1 and…
Fluorescence Microscopy-Based RNA Interference Screening
Plate type Advantage Drawback Proposed application 96-Well plate Large number of cells per well, area ~30 mm2 Low number of wells Validation screen with preselected low number of targets 384-Well…
