High-Pressure Fluorescence Applications
(1) INSERM U554, Centre de Biochimie Structurale, Montpellier, France (2) Centre de Biochimie Structurale, INSERM U554, Montpellier, France Abstract Fluorescence is the most widely used technique to study the…
GENERAL SURGERY
Upconversion Spectrophotofluorometry
(1) NIDDK, National Institutes of Health, Bethesda, MD, USA (2) NHLBI, National Institutes of Health, Bethesda, MD, USA Abstract As the other chapters attest, sensitivity of fluorescent molecules to…
Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family
(1) Faculté de Parmacie, Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Illkirch, France Abstract Environment-sensitive fluorescent membrane probes are attractive tools for investigating the membrane…
Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope
(1) Laboratory of General Chemistry and Physical Pharmacy, Ghent University and Centre of Nano- and Biophotonics, Ghent, Belgium (2) Laboratory of General Chemistry and Physical Pharmacy, Ghent University, Ghent, Belgium…
Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells
(1) Faculty of Science, Department of Chemistry, National University of Singapore, Singapore, Singapore (2) Department of Chemistry, Faculty of Science, National University of Singapore, Singapore, Singapore (3) Mechanobiology Institute, Singapore,…
Biosynthetic Incorporation of Tryptophan Analogs in Proteins
(1) Laboratory of Biophysical Chemistry and Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, Groningen, The Netherlands Abstract Biosynthetic incorporation of Trp analogs in a protein can help…
Data Management
2: Male9AgeDocument218–6599Age GroupRecode of Age11: 18–352: 36–503: 51–659FBSFasting Blood Glucose3Number 50–450999 Note that we have used 9, 99, and 999 as missing value codes. Such codes are common, but it…
Acquiring High Quality Data
html xmlns=”http://www.w3.org/1999/xhtml” xmlns:mml=”http://www.w3.org/1998/Math/MathML” xmlns:epub=”http://www.idpf.org/2007/ops”> 29 Acquiring High Quality Data The validity of a study depends on many things, from hypotheses that are based on sound science, to a study design…
Blinding in Observational Studies
html xmlns=”http://www.w3.org/1999/xhtml” xmlns:mml=”http://www.w3.org/1998/Math/MathML” xmlns:epub=”http://www.idpf.org/2007/ops”> 28 Blinding in Observational Studies Although blinding is typically thought of as something done in interventional studies, it can and should be used in observational studies…
Matching in Observational Studies
html xmlns=”http://www.w3.org/1999/xhtml” xmlns:mml=”http://www.w3.org/1998/Math/MathML” xmlns:epub=”http://www.idpf.org/2007/ops”> 27 Matching in Observational Studies In Sections 25.4 and 26.3.1 we described the use of multiple cohorts and multiple control groups, respectively. For cohort studies, cohort…
