Fig. 9.1 Analytical characterization of IgG1 monoclonal antibody solutions containing soluble aggregates measured by orthogonal methods, SE-HPLC (a, b, c) and SV-AUC (d, e, f). Protein aggregates of varying size…
Fig. 5.1 (a) Far-UV CD spectra of protein 1–4 (spectral similarity compared to that of protein 1 is 100% protein 1; 5.0% protein 2; 44.9% protein 3; 28.7% protein 4)….
Fig. 2.1 Representative differential scanning fluorometry spectra obtained with a therapeutic protein on 96-well plate filled with different formulations for each well. Fluorescence intensity in relative fluorescence units (RFU) is…
Fig. 10.1 Microparticle analysis procedure The majority of the microparticles can be isolated through filtration using filter appropriate for the planned analysis. A gold-coated membrane filter (Rapid-ID, Berlin, Germany) facilitates…
Instabilities 1. Physical • Conformational stability Thermal denaturation Cold denaturation Pressure-induced denaturation Chemical denaturation • Colloidal stability • Surface/interfacial-induced denaturation 2. Chemical • Deamidation • Asp isomerization • Fragmentation •…
Alexander PA, He Y, Chen Y, Orban J, Bryan PN (2007) The design and characterization of two proteins with 88 % sequence identity but different structure and function. Proc Natl…
Narhi LO, Jiang Y, Deshpande R, Kang S, Shultz J (2010) Approaches to control protein aggregation during bulk production. In: Wang W, Roberts CJ (eds) Aggregation of therapeutic proteins. Wiley,…
Method category Method Size Pros and cons Methods references Size-exclusion chromatography HPSEC <0.1 μm Pros: fast, high throughput Cons: limited size range, limited choice of buffers, possible matrix interactions Liu…
–-damage formulation, where only the anisotropic elastic part is assumed to damage. The resulting coupled, highly non-linear system of equations is symmetric and can conveniently be solved by standard incremental-iterative…
Nonlinear complex dynamical system 1 Introduction Flow affects the blood vessel responses in many ways, through mechanotransduction mechanisms, or by modifying the interactions between blood-borne agonists and cell receptors, especially…
