Standard dilutions Standard estradiol, ng/mL Volume of standard added, μL Volume of solution, mL Concentration, ng/mL Dilution 1 10,00,000 40 10 4000 Dilution 2 4000 50 10 20 Calibration standard…
Fig. 1 HPLC-ESI-MS/MS ion chromatogram of GABA 1 (m/z 104.1 > 87.1), GABA 2 (m/z 104.1 > 69.1), GABA-D2 (m/z 106.0 > 89.1). Concentration of GABA shown is 169 nM 3.2 Sample Preparation (Total GABA…
Calibrator Cys (μM) Phe, Tyr, Gly, PEA (μM) Blank 0 0 Cal 1 15.6 31.2 Cal 2 31.2 62.5 Cal 3 62.5 125 Cal 4 125 250 Cal 5 250…
2°(μl) 3° (μl) RBC matrix Final conc. (μM) Cal 1 100 q.s. to 10 ml 10 Cal 2 100 q.s. to 10 ml 100 Cal 3 200 q.s. to…
Calibrator number Volume of previous calibration material (ml) Volume of deionized water (ml) Final concentration (pg/ml) 6 3.75 (working stock) 2.25 625 5 2.0 (Cal #6) 4.0 208 4 2.0…
(1) Departement of Pathology, University of Michigan Health System, Ann Arbor, MI 48109-5054, USA (2) Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 2nd Street SW, Rochester, MN 55905, USA …
Calibrator Volume of standard stock solution (mL) Volume of special reagent water (mL) Final concentration (μmol/L) 1 1.0 (ADMA stock solution 2) 1.0 (SDMA stock solution 2) 2.0 (Arginine stock…
Fig. 1 Schematic diagram of a mass spectrometry analysis 2.1 GC-MS GC-MS has been used in clinical laboratories for several decades. A typical GC-MS uses helium gas, sample injection port,…
© Springer Science+Business Media New York 2016Uttam Garg (ed.)Clinical Applications of Mass Spectrometry in Biomolecular AnalysisMethods in Molecular Biology137810.1007/978-1-4939-3182-8_7 7. Sensitive, Simple, and Robust Nano-Liquid Chromatography-Mass Spectrometry Method for Amyloid Protein Subtyping Drew Payto1, Courtney Heideloff1…
Days Methylene blue Bromo phenol blue Congo red Malachite green Rose bengal MB MB MB BPB BPB BPB CR CR CR MG MG MG RB RB RB 1 mg 2 mg 3 mg…
