7 % BSA (mL) 1° Standard (μL) PHE/TYR 2° Standard (μL) PHE/TYR Calibrator (mg/dL) PHE/TYR 0.940 –/– 50/10 0.5/0.1 0.930 20/– –/50 2.0/0.5 0.850 50/– –/100 5.0/1.0 0.880 100/20 –/–…
Compound Concentration (nmol/mL) D9-l-Carnitine (C0) 0.760 D3-Acetycarnitine (C2) 0.190 D3-Propionylcanitine (C3) 0.038 D3-Butyrylcarnitine (C4) 0.038 D9-Isovalerylcarnitine (C5) 0.038 D3-Octanoylcarnitine (C8) 0.038 D9-Tetradecanoylcarnitine (C14) 0.038 D3-Hexadecanoylcarnitine (C16) 0.076 2. Quality…
Fig. 1 HPLC -ESI-MS/MS ion chromatogram of 5-MTHF 100 nM standard. [5-MTHF 1 (m/z 460.3 > 313.3), 5-MTHF 2 (m/z 460.3 > 194.5), and 5-MTHF-13C5 (m/z 465.3 > 313.3)] Fig. 2 HPLC -ESI-MS/MS ion chromatogram of…
Std level (μU/mL) Volume of 100 μU/mL mixed calibrator (μL) Volume of serum pool ( μL) 2.5 12.5 487.5 5 25 475 10 50 450 25 125 375 50 250 250…
To make: Use source solution Volume of source solution (mL) Volume of CLRW Metanephrine concentration (ng/mL)a Normetanephrine concentration (ng/mL)a Std 5 Spiked from Stock 160 μL QS to 100 mL…
Ion Mass (amu) Charge state Mass accuracy IGF-1 analyte 1093.5209 +7 10 ppm 1093.3475 +7 10 ppm 1093.6640 +7 10 ppm Rat IGF-1Ox IS 1098.9622 +7 10 ppm Compound parameters…
Fig. 1 Typical elution profile for sphingolipids analysis by LC/MS/MS 2 Materials and Equipment 2.1 Samples Venous blood is drawn into Vacutainer® tubes containing EDTA (Becton, Dickinson and Company, Franklin…
Analyte Transition Dwell (s) Cone (V) Collision (eV) Uracil 112.8 > 70 0.25 24 14 15N2-Uracil 114.8 > 70.7 0.25 24 15 Dihydrouracil 114.8 > 72.9 0.25 25 11 13C4,15N-Dihydrouracil 121.0 > 76.7 0.25 26 10 Thymine 127 > 110.2…
Standard dilutions Standard estradiol, ng/mL Volume of standard added, μL Volume of solution, mL Concentration, ng/mL Dilution 1 10,00,000 40 10 4000 Dilution 2 4000 50 10 20 Calibration standard…
Calibrator level Spiking solution Volume of spiking solution (μL) Final volume (mL) Final concentration (ng/dL) Final concentration (pmol/L) 1 Low 35 50 1.6 45 2 Low 80 50 3.7 104…
