Fig. 28.1 Renal amyloid deposits—Congo red stains viewed under polarized light. (a) Glomerular and extraglomerular vessels and interstitial amyloid. (b) Glomerular deposits. (c) Interstitial amyloid. (d) Amyloid in the medullary…
Shotgun proteomics Targeted proteomics Tries to identify every protein in a mixture Tries to identify specific protein targets Analogous to gene expression profiling Analogous to RTPCR or immunoassays Semiquantitative Quantitative…
Fig. 10.1 Nodular glomerulosclerosis secondary to κ-LCDD. (a) X500 and (b) X750. (a) Hematoxylin and eosin and (b) immunohistochemical stain for κ-light chains. (a) The glomerulus displays the typical nodularity—nodular…
Fig. 32.1 Gross appearances of lymph node involvement in AL amyloidosis. Cut surface of the lymph node involved by AL amyloidosis shows replacement of the parenchyma with a waxy appearing…
Fig. 33.1 Systemic ATTR amyloidosis involving the lung. Eosinophilic amorphous material consistent with vascular amyloid deposition in the lung (a, H&E, arrow) stained with Congo red (b, arrow). Congo red…
Fig. 31.1 Shown are the characteristic features of amyloid deposition in peripheral sural nerve biopsy tissue studied by light microscopy. Paraffin-stained sections show an acellular and homogenous proteinaceous material on…
Fig. 20.1 A case of AL-lambda. (a) Stain for lambda light chain. Notice the high signal-to noise ratio. (b) Stain for kappa light chain. Although the exposure had to be…
Fig. 14.1 Diagnosis of amyloid in fixed tissues after CR staining, as viewed under three different types of illumination (“triple illumination”), in order to demonstrate the detection of amyloid and…
Amyloid class No. of patients % of total ALλ 271 43.3 ALκ 118 18.8 (AL, sum) (389) (62.1) ATTR 93 14.9 AA 80 12.8 AFib 14 2.2 Aβ2M 11 1.8…
Fig. 24.1 Virtual 2D map, showing the distribution of proteins identified by LC-MS in a representative subcutaneous fat tissue aspirate, according to isoelectric point and molecular weight. The inset and…
