Fig. 1 TIRF versus epifluorescence imaging. A fixed MDA-MB-231 breast cancer cell labeled for alpha-tubulin (a, b) and phospho-paxillin (c, d). TIRF imaging (a, c) results in a much higher…
Fig. 1 FCCS setup. Light from two laser beams is focused into the sample using a highly corrected objective lens with a large numerical aperture. Fluorescence is guided via a…
Fig. 1 Principle of single-molecule super-resolution microscopy. (a) Photoswitchable fluorophores are transferred from a fluorescent bright state into a nonfluorescent dark state by the irradiation of light. (b) By activating…
Fig. 1 Moiré fringes. (a) and (b) are two examples of fine patterns. When one is superimposed onto the other, a coarser beat pattern—moiré fringes—appears (c) In optics, reciprocal space…
Fig. 1 Signals detected by ratio imaging and FilterFRET. (a–c) Spectra of a perfect FRET pair, i.e., there is no leak-through and cross-excitation between the fluorophores. (d–f) Spectra of real-world…
Here, I is the maximum intensity of the depletion beam, and the saturation intensity I S is the intensity required to reduce the fluorescence probability by half [4]. Thus, larger…
Fig. 1 Principles of 2PE microscopy. (a) Jablonski energy diagram showing the electron excitation process in single- (left ) and two-photon (right ) fluorescence microscopy. Single-photon excitation (left ) requires the absorption of…
Fig. 1 4Pi setup. (a) Scheme of the commercially available 4Pi setup (TCS 4Pi microscope, type A, Leica Microsystems, Mannheim, Germany). O1 and O2: objective lenses; S: sample; BS: beamsplitter;…
Fig. 1 Cartoon diagram of the photoactivatable Rac (PA-Rac). A LOV domain and its C-terminal Jα helix are fused to the N-terminus of a constitutively active mutant of Rac1 and…
Plate type Advantage Drawback Proposed application 96-Well plate Large number of cells per well, area ~30 mm2 Low number of wells Validation screen with preselected low number of targets 384-Well…
