Ion Mass (amu) Charge state Mass accuracy IGF-1 analyte 1093.5209 +7 10 ppm 1093.3475 +7 10 ppm 1093.6640 +7 10 ppm Rat IGF-1Ox IS 1098.9622 +7 10 ppm Compound parameters…
Fig. 1 Typical elution profile for sphingolipids analysis by LC/MS/MS 2 Materials and Equipment 2.1 Samples Venous blood is drawn into Vacutainer® tubes containing EDTA (Becton, Dickinson and Company, Franklin…
Analyte Transition Dwell (s) Cone (V) Collision (eV) Uracil 112.8 > 70 0.25 24 14 15N2-Uracil 114.8 > 70.7 0.25 24 15 Dihydrouracil 114.8 > 72.9 0.25 25 11 13C4,15N-Dihydrouracil 121.0 > 76.7 0.25 26 10 Thymine 127 > 110.2…
Calibrator level Spiking solution Volume of spiking solution (μL) Final volume (mL) Final concentration (ng/dL) Final concentration (pmol/L) 1 Low 35 50 1.6 45 2 Low 80 50 3.7 104…
Time (min) Total flow (mL/min) Pump B concentration (%) 0 5 0.4 0.6 5 0.5 0.6 50 1.9 0.6 50 2.0 0.6 100 2.6 0.6 100 2.8 1.0 100 2.9…
Standard dilutions Standard estradiol, ng/mL Volume of standard added, μL Volume of solution, mL Concentration, ng/mL Dilution 1 10,00,000 40 10 4000 Dilution 2 4000 50 10 20 Calibration standard…
Fig. 1 HPLC-ESI-MS/MS ion chromatogram of GABA 1 (m/z 104.1 > 87.1), GABA 2 (m/z 104.1 > 69.1), GABA-D2 (m/z 106.0 > 89.1). Concentration of GABA shown is 169 nM 3.2 Sample Preparation (Total GABA…
Calibrator Cys (μM) Phe, Tyr, Gly, PEA (μM) Blank 0 0 Cal 1 15.6 31.2 Cal 2 31.2 62.5 Cal 3 62.5 125 Cal 4 125 250 Cal 5 250…
2°(μl) 3° (μl) RBC matrix Final conc. (μM) Cal 1 100 q.s. to 10 ml 10 Cal 2 100 q.s. to 10 ml 100 Cal 3 200 q.s. to…
(1) Departement of Pathology, University of Michigan Health System, Ann Arbor, MI 48109-5054, USA (2) Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 2nd Street SW, Rochester, MN 55905, USA …
