Analyte
Transition
Dwell (s)
Cone (V)
Collision (eV)
Uracil
112.8 > 70
0.25
24
14
15N2-Uracil
114.8 > 70.7
0.25
24
15
Dihydrouracil
114.8 > 72.9
0.25
25
11
13C4,15N-Dihydrouracil
121.0 > 76.7
0.25
26
10
Thymine
127 > 110.2
0.25
26
13
D4-Thymine
131 > 114.1
0.25
35
16
Dihydrothymine
129 > 69
0.25
22
14
D9-Dihydrothymine
135 > 74
0.25
28
15
Thymidine
243.2 > 127.2
0.25
10
12
D4-Thymidine
247.2 > 131.2
0.25
15
10
(b)
Capillary: 2.9
(c)
Extractor: 2.0
(d)
Desolvation temperature: 400
3.2 Data Analysis
1.
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A sample of MRM chromatograms of purine metabolites is shown in Fig. 1. Normal reference ranges are listed in Table 2 and diagnostic markers for pyrimidine deficiencies in Table 3.
Fig. 1
LCMS chromatograms of pyrimidine metabolites. From the top, thymidine , dihydrothymine , thymine , dihydrouracil , and uracil . Note that thymine elutes at 5.52 min. A contamination with the same 127 > 110.2 MRM is eluted later at 8.99 min
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