(1) Swammerdam Institute for Life Sciences, van Leeuwenhoek Centre for Advanced Microscopy, Section of Molecular Cytology, University of Amsterdam, Amsterdam, The Netherlands Abstract Nowadays, fluorescent protein (FP) variants have…
(1) Department of Biotechnology & Biophysics, Biozentrum, Julius-Maximilians-University Würzburg, Würzburg, Germany Abstract Quenching of organic fluorophores by aromatic amino acids and DNA nucleotides with expelled electron donating properties allows…
(1) SUPA School of Physics and Astronomy, University of St. Andrews, St. Andrews, Scotland, UK (2) Faculté des Sciences, RNA Group, Département de Biologie, Université de Sherbrooke, Sherbrooke, QC, Canada…
(1) Laboratoire d’Optique et Biosciences, INSERM U696-CNRS UMR7645, Ecole Polytechnique, Palaiseau, France (2) Institut für Physikalische und Theoretische Chemie, Julius-Maximilians-Universität Würzburg, Würzburg, Germany (3) Department of Biophysics, Medical School, University…
(1) BIOTEC, Biophysics, Technische Universität Dresden, Dresden, Germany (2) Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, Germany Abstract Scanning fluorescence correlation spectroscopy (SFCS) with a scan…
(1) Department of Chemistry, KU Leuven, Leuven, Belgium Abstract In the last two decades, fluorescent proteins became an indispensable tool to noninvasively label a protein in living cells. The…
(1) Fluorescence Division, HORIBA Instruments Inc., Edison, NJ, USA Abstract Contemporary spectrofluorimeters comprise exciting light sources, excitation and emission monochromators, and detectors that without correction yield data not conforming…
(1) BIOTEC, Biophysics, Technische Universität Dresden, Dresden, Germany (2) Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, Germany Abstract Fluorescence correlation spectroscopy (FCS) can add dynamic molecular…
, James Levitt1 and Pei-Hua Chung1, 2 (1) Department of Physics, King’s College London, London, UK (2) Faculty of Life Sciences, University of Manchester, Manchester, UK Abstract Fluorescence can be characterized…
(1) Multidimensional Microscopy and Cellular Diagnostics, Medical Faculty, Otto-von-Guericke-University, Magdeburg, Germany (2) Special Laboratory Electron- and Laserscanning Microscopy, Leibniz Institute for Neurobiology (LIN), Magdeburg, Germany Abstract Fluorescence lifetime imaging…
