Nanometrology
and Philip Yip1 (1) The Photophysics Research Group, Department of Physics, Centre for Molecular Nanometrology, SUPA, University of Strathclyde, Glasgow, Scotland, UK Abstract Methods and protocols are described when using…
Quantitative Study of Protein–Protein Interactions in Live Cell by Dual-Color Fluorescence Correlation Spectroscopy
and Marc Tramier1 (1) Institut de Génétique et Développement de Rennes, UMR 6290, CNRS, Université de Rennes 1, Rennes, France (2) Institut Jacques Monod, UMR 7592, CNRS, Université Paris-Diderot, Paris, France…
MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes
and Jose R. Tusell1 (1) Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, USA Abstract Principles behind quenching of tryptophan (Trp) fluorescence are updated and extended in light of…
A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging
, Eric J. Rees1 and Gabriele S. Kaminski Schierle1 (1) Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK Abstract Förster resonance energy transfer (FRET) has become one of the most ubiquitous…
Z-Scan Fluorescence Correlation Spectroscopy as a Tool for Diffusion Measurements in Planar Lipid Membranes
(1) Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic (2) J. Heyrovský Institute of Physical Chemistry v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech…
Global Analysis of FRET–FLIM Data in Live Plant Cells
(1) Laboratoire d’Optique et Biosciences, INSERM U696-CNRS UMR7645, Ecole Polytechnique, Palaiseau, France (2) Faculty of Sciences, Department of Physics and Astronomy, VU University Amsterdam, Amsterdam, The Netherlands (3) Laboratory of…
Implementation and Application of Pulsed Interleaved Excitation for Dual-Color FCS and RICS
(1) Physical Chemistry, Department of Chemistry, Munich Center for Integrated Protein Science (CiPSM) and Center for Nanoscience (CeNS), Ludwig-Maximilian-Universität München, Munich, Germany (2) Loomis Laboratory of Physics, Department of Physics,…
Frequency Domain Fluorometry: Theory and Application
(1) Department Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA Abstract Frequency domain fluorometry is a widely utilized tool in the…
Multimodal Fluorescence Imaging Spectroscopy
(1) Nanobiophysics Group, University of Twente, Enschede, The Netherlands Abstract Multimodal fluorescence imaging is a versatile method that has a wide application range from biological studies to materials science….
Steady-State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions
(1) Department Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA Abstract Fluorescence methods are often employed for the characterization of molecular…
