High-Grade Undifferentiated Carcinoma

Antibody

Prostate

Urothelial

PSA

68-94%

0%

PAP

78-95%

0%

PSMA

> 95% (best)

0%

HMCK(34βE12)

6-10%

65-100%

Uroplakin-3

0%

57-60%

Thrombomodulin

0%

49-69%

p63

0-18%

70-75%

CD57

94%

17%

S100p

0-1%

78-86%

GATA3

0-3%

67%

Enteric-type Adenocarcinoma

Antibody

Bladder

Colon

CK7(+)/CK20(+)

24%

8%

CK7(+)/CK20(-)

41%

0%

CK7(-)/CK20(+)

29%

82%

CK7(-)/CK20(-)

6%

10%

CDX-2

47-100%

99-100%

β-catenin

0%

81% (best)

Villin

65-100%

82-98%

Thrombomodulin

59%

0%

Spindle Cell Proliferations: Adults

Antibody

Pseudosarcomatous Myofibroblastic Proliferation

Sarcomatoid Carcinoma

Leiomyosarcoma

Actin-sm

63-100%

15-80%

43-100%

Desmin

27-80%

0-40%

0-60%

h-caldesmon

0-66%

ND

100%

PAN-CK(AE1/AE3)

36-89%

67-100%

0-38%

EMA/MUC1

0-50%

50-100%

0-12%

ALK1

75-89%

0%

10%

p63

0%

50%

23%

HMCK(34βE12)

0%

25-27%

0%

Spindle Cell Proliferations: Children

Antibody

Pseudosarcomatous Myofibroblastic Proliferation

Rhabdomyosarcoma

Actin-sm

63-100%

97%

Desmin

27-80%

97%

MyoD1

0%

100%

Myogenin

0%

100%

ALK1

75-89%

20%

Bladder Neoplasms with Nested Morphology

Antibody

Paraganglioma

Carcinoid

Urothelial Carcinoma

Melanoma

PAN-CK(AE1/AE3)

–

+

+

–

Synaptophysin

+

+

–

–

Chromogranin

+

+

–

–

S100

+ (sustentacular cells)

–

–

+

ND – No Data

Flat Urothelial Lesions with Atypia (URO3 Cocktail)

Antibody

Normal

Reactive

CIS

CK20 (cytoplasmic, red)

Umbrella layer only

Umbrella layer only

Full thickness urothelium

CD44 (membranous, brown)

Basal layer only

Increased expression in intermediate cells to full thickness urothelium

Basal layer only or loss of expression

p53 (nuclear, brown)

Rare cells; weak reactivity

Rare cells; weak reactivity

Diffuse, strong reactivity

URO3 is a antibody cocktail comprised of three antibodies: CK20, CD44, and p53.