1. List the media of choice for optimal recovery and cultivation of Francisella tularensis. 2. Describe the optimal incubation conditions for F. tularensis. 3. Describe the normal habitat and means of transmission of Francisella spp. 4. Describe the symptoms of tularemia and differentiate the various clinical presentations, including ulceroglandular, glandular, oculoglandular, oropharyngeal, systemic (typhoidal), and pneumonic tularemia. Organisms belonging to the genus Francisella are faintly staining, tiny, gram-negative coccobacilli that are oxidase and urease negative, catalase-positive, nonmotile, non–spore forming, strict aerobes. The taxonomy of this genus continues to be in flux. Current members of the genus share greater than 97% identity based on 16SrRNA sequence analysis. The most current proposed taxonomy is summarized in Table 38-1. For the most part, different subspecies are associated with different geographic regions. TABLE 38-1 Most Recent Taxonomy of the Genus Francisella and Key Characteristics The disease associated with F. tularensis, known as tularemia, is recognized worldwide. In the United States the clinical manifestations have been referred to as rabbit fever, deer fly fever, and market men’s disease. The clinical manifestation depends on the mode of transmission, the virulence of the infecting organism, the immune status of the host, and the length of time from infection to diagnosis and treatment. The typical clinical presentation after inoculation of F. tularensis through abrasions in the skin or by arthropod bites includes the development of a lesion at the site and progresses to an ulcer; lymph nodes adjacent to the site of inoculation become enlarged and often necrotic. Once the organism enters the bloodstream, patients become systemically ill with high temperature, chills, headache, and generalized aching. Clinical manifestations of infection with F. tularensis range from mild and self-limiting to fatal; they include glandular, ulceroglandular, oculoglandular, oropharyngeal, systemic, and pneumonic forms. These clinical presentations are briefly summarized in Table 38-2. TABLE 38-2 Clinical Manifestations of Francisella tularensis Infection Specimen collection for the identification of F. tularensis is highly dependent on the type of clinical manifestation. A detailed description of the recommended type of specimen associated with the patient’s clinical presentation is presented in Table 38-3. In light of recent events and concerns about bioterrorism, laboratories must keep in mind that isolation of F. tularensis from blood cultures might be considered a potential bioterrorist attack; F. tularensis is considered one of the Select Biological Agents of Human Disease (see Chapter 80). TABLE 38-3 Recommended Specimen Type Based on Clinical Manifestation
Francisella
General Characteristics
Organism
Primary Region
Disease in Humans
Requires Cystine/Cysteine
F. tularensis subsp. tularensis
North America (United States and Canada)
Most severe: Tularemia (all forms, see Table 38-2)
+
F. tularensis subsp. holartica
Europe, former Soviet Union, Japan, North America
Least severe: Tularemia (all forms)
+
F. tularensis subsp. mediasiatica
Kazakhstan, Uzbekistan
Severe: Tularemia
+
F. noatunensis (formerly F. philomiragia subsp. noatunensis)
North and South America
Emerging pathogen of fish; no human infections identified
_
F. novicida
North America
Mild illness; virulent in immunocompromised patients
_
F. philomiragia (formerly Yersinia philomiragia)
North America
Mild illness; virulent only in immunocompromised individuals and near-drowning victims
_
Spectrum of Disease
Types of Infection
Clinical Manifestations and Description
Ulceroglandular
Common; ulcer and lymphadenopathy; rarely fatal
Glandular
Common; lymphadenopathy; rarely fatal
Oculoglandular
Conjunctivitis, lymphadenopathy
Oropharyngeal
Ulceration in the oropharynx
Systemic (typhoidal) tularemia
Acute illness with septicemia; 30% to 60% mortality rate; no ulcer or lymphadenopathy
Pneumonic tularemia
Acquired by inhalation of infectious aerosols or by dissemination from the bloodstream; pneumonia; most serious form of tularemia
Laboratory Diagnosis
Specimen Collection, Transport, and Processing
CLINICAL MANIFESTATION
Ulceroglandular
Glandular
Oculoglandular
Oropharyngeal
Typhoidal
Pneumonic
Whole blood
X
X
X
X
X
X
Serum
X
X
X
X
X
X
Pharyngeal swabs, bronchial/tracheal washes or aspirates, sputum, transthoracic lung aspirates, and pleural fluid
X
X
X
Swabs from visible lesions
X
X
Aspirates from lymph nodes or lesions
X
X
X
Stay updated, free articles. Join our Telegram channel
Full access? Get Clinical Tree
Francisella
![](https://clinicalpub.com/wp-content/uploads/2023/09/banner1.png)