Effects of Soybean, Sunflower, Olive and Sugar Cane Wax Oils and their Respective Fatty Acids in Cutaneous Inflammation


Sugarcane


Sunflower


Soybean


Olive


Capric (C10)


0.33





Lauric (C12)


0.45





Miristic (C14)


0.56



0.34



Pentadecanoic (C15)


0.03





Palmitic (C16)


29.40


15.41


13.91


18.44


Palmitoleic (C16:1)


0.02




2.65


Estearic (C18)


3.73


3.23


4.06


2.59


Oleic (C18:1)


14.88


26.36


23.56


65.25


Linoleic (C18:2)


42.20


55.00


52.74


11.07


Linolenic (C18:3)


8.03



5.39



Araquidic (C20)


0.10





Dodeicosanoic (C22)


0.12





Trieicosanoic (C23)


0.02





Tetraeicosanoic (C24)


0.11





Pentaicosanoic (C25)


0.02





Assay of PLA2 activity


Ear tissues from mice killed 18 hours after TPA treatment were homogenized in 0.5 ml homogenizing buffer (100 mM KCl, 10 mM tris-HCl, pH 7.4 (buffer A). Homogenates were centrifuged for 15 min at 3500g and 4°C and pellet was eliminated. 50 ml of each sample (20-25 mg of protein) were incubated at 24°C, 15 min with buffer A plus 2 mM CaCl2, and with fluorescence substrate 1 acyl-2-[6-[(7-nitro-1,2,3 benzoxadiazol-4-il)amino]-caproil]phosphatidilcoline (5×106 M) (Molecular Probes, Eugene, OR). Fluorescence excitation was at 470nm and emission at 540 nm. Titers were based on comparisons with standard curves obtained with bovine pancreas phospholipase A2 of known activity and the determination were performed in a spectrofluorimeter (Wittenauer et al., 1984).


Mieloperoxidase Assay


Ear tissues from mice killed 6 hours after TPA treatment were homogenized in 50 mM K2HPO4/KH2PO4 buffer (pH 6) containing 0.5 per cent of hexadecyl trimethylammonium bromide using a polytron homogenizer. After freeze-thawing 3 times, the samples were centrifuged at 2500g for 30 min at 4°C and the resulting supernatant assayed spectrophotometrically for MPO as described before (Romay et al., 1998).


Psoriasis Tail Test


The modified mouse tail test established by Bosman et al

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May 21, 2017 | Posted by in PHARMACY | Comments Off on Effects of Soybean, Sunflower, Olive and Sugar Cane Wax Oils and their Respective Fatty Acids in Cutaneous Inflammation

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