Sugarcane

Sunflower

Soybean

Olive

Capric (C10)

0.33

–

–

–

Lauric (C12)

0.45

–

–

–

Miristic (C14)

0.56

–

0.34

–

Pentadecanoic (C15)

0.03

–

–

–

Palmitic (C16)

29.40

15.41

13.91

18.44

Palmitoleic (C16:1)

0.02

–

–

2.65

Estearic (C18)

3.73

3.23

4.06

2.59

Oleic (C18:1)

14.88

26.36

23.56

65.25

Linoleic (C18:2)

42.20

55.00

52.74

11.07

Linolenic (C18:3)

8.03

–

5.39

–

Araquidic (C20)

0.10

–

–

–

Dodeicosanoic (C22)

0.12

–

–

–

Trieicosanoic (C23)

0.02

–

–

–

Tetraeicosanoic (C24)

0.11

–

–

–

Pentaicosanoic (C25)

0.02

–

–

–

Assay of PLA₂ activity

Ear tissues from mice killed 18 hours after TPA treatment were homogenized in 0.5 ml homogenizing buffer (100 mM KCl, 10 mM tris-HCl, pH 7.4 (buffer A). Homogenates were centrifuged for 15 min at 3500g and 4°C and pellet was eliminated. 50 ml of each sample (20-25 mg of protein) were incubated at 24°C, 15 min with buffer A plus 2 mM CaCl₂, and with fluorescence substrate 1 acyl-2-[6-[(7-nitro-1,2,3 benzoxadiazol-4-il)amino]-caproil]phosphatidilcoline (5×10^–6 M) (Molecular Probes, Eugene, OR). Fluorescence excitation was at 470nm and emission at 540 nm. Titers were based on comparisons with standard curves obtained with bovine pancreas phospholipase A₂ of known activity and the determination were performed in a spectrofluorimeter (Wittenauer et al., 1984).

Mieloperoxidase Assay

Ear tissues from mice killed 6 hours after TPA treatment were homogenized in 50 mM K₂HPO₄/KH₂PO₄ buffer (pH 6) containing 0.5 per cent of hexadecyl trimethylammonium bromide using a polytron homogenizer. After freeze-thawing 3 times, the samples were centrifuged at 2500g for 30 min at 4°C and the resulting supernatant assayed spectrophotometrically for MPO as described before (Romay et al., 1998).

Psoriasis Tail Test

The modified mouse tail test established by Bosman et al

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