Mycosis Fungoides
Mycosis Fungoides Amy C. Musiek Alejandro A. Gru András Schaffer DEFINITION Mycosis fungoides (MF) is the most common form of primary cutaneous T-cell lymphoma (CTCL) characterized by epidermotropic small-to-medium–sized T…
PATHOLOGY & LABORATORY MEDICINE
Approach to the Diagnosis of Cutaneous T-Cell Lymphomas
Approach to the Diagnosis of Cutaneous T-Cell Lymphomas Alejandro A. Gru András Schaffer INTRODUCTION The diagnosis of cutaneous T-cell lymphomas (CTCLs) requires a careful evaluation of the clinical features, as…
Next-Generation Sequencing
Next-Generation Sequencing Ian S. Hagemann DNA SEQUENCING IN HISTORICAL PERSPECTIVE Maxam–Gilbert Sequencing Early DNA sequencing methods took advantage of chemical reactions that selectively degrade DNA at specific nucleotides. For example,…
Fluorescent In Situ Hybridization and Other Molecular Techniques in the Diagnosis of Cutaneous Lymphomas
Fluorescent In Situ Hybridization and Other Molecular Techniques in the Diagnosis of Cutaneous Lymphomas Andrea L. Salavaggione Alejandro A. Gru FLUORESCENT IN SITU HYBRIDIZATION In situ hybridization (ISH) allows for…
PCR-Based Gene Rearrangement Studies
PCR-Based Gene Rearrangement Studies Ian S. Hagemann GENE REARRANGEMENT IN LYMPHOCYTE DEVELOPMENT Immunoglobulin Rearrangement Human B-cell antigen receptor genes include the immunoglobulin (Ig) heavy-chain locus (IGH) at 14q32, the kappa…
Flow Cytometric Analysis in Hematopathology Diagnosis
Flow Cytometric Analysis in Hematopathology Diagnosis Gerard Lozanski Alejandro A. Gru INTRODUCTION Multicolor flow cytometric analysis (FCA) is routinely used in diagnostic hematopathology. FCA allows for rapid and accurate characterization…
Molecular Biology of T-Cell Lymphoma
Molecular Biology of T-Cell Lymphoma Anjali Mishra Pierluigi Porcu INTRODUCTION T-cell lymphomas represent a group of lymphoproliferative disorders of T-cell origin. T-cell lymphomas tend to be rare, heterogeneous, and more…
Mononuclear Phagocytes of the Skin
Mononuclear Phagocytes of the Skin Sena J. Lee András Schaffer INTRODUCTION The mononuclear phagocyte system (MPS) is composed of at least three subsets of tissue histiocytes including monocytes, macrophages, and…
NK Cell Immunobiology
NK Cell Immunobiology Aharon G. Freud INTRODUCTION Natural killer (NK) cells are innate lymphoid cells (ILCs) that have a characteristic large granular lymphocyte morphology and that are able to recognize…
Cutaneous B-Cell Immunobiology
Cutaneous B-Cell Immunobiology Alejandro A. Gru INTRODUCTION Originally termed skin-associated lymphoid tissue (SALT), the “skin immune system” (SIS) comprises specialized skin-resident immune cells, as well as immunocompetent skin-trophic lymphocytes constantly…
