Primary Cutaneous CD4+ Small/Medium T-Cell Lymphoma Sanam Loghavi Michael T. Tetzlaff DEFINITION As its name implies, primary cutaneous small/medium CD4+ T-cell lymphoma is a cutaneous T-cell lymphoma confined to the…
Primary Cutaneous CD30+ T-Cell Lymphoproliferative Disorders Rebecca L. King Amy C. Musiek Andrew L. Feldman INTRODUCTION Primary cutaneous CD30+ T-cell lymphoproliferative disorders (TLPDs) are challenging to both pathologists and clinicians…
Sézary Syndrome Ellen J. Kim András Schaffer DEFINITION Sézary syndrome (SS) is a rare subtype of cutaneous T-cell lymphoma (CTCL) that is closely related to, but not identical to, mycosis…
Mycosis Fungoides Amy C. Musiek Alejandro A. Gru András Schaffer DEFINITION Mycosis fungoides (MF) is the most common form of primary cutaneous T-cell lymphoma (CTCL) characterized by epidermotropic small-to-medium–sized T…
Approach to the Diagnosis of Cutaneous T-Cell Lymphomas Alejandro A. Gru András Schaffer INTRODUCTION The diagnosis of cutaneous T-cell lymphomas (CTCLs) requires a careful evaluation of the clinical features, as…
Next-Generation Sequencing Ian S. Hagemann DNA SEQUENCING IN HISTORICAL PERSPECTIVE Maxam–Gilbert Sequencing Early DNA sequencing methods took advantage of chemical reactions that selectively degrade DNA at specific nucleotides. For example,…
Fluorescent In Situ Hybridization and Other Molecular Techniques in the Diagnosis of Cutaneous Lymphomas Andrea L. Salavaggione Alejandro A. Gru FLUORESCENT IN SITU HYBRIDIZATION In situ hybridization (ISH) allows for…
PCR-Based Gene Rearrangement Studies Ian S. Hagemann GENE REARRANGEMENT IN LYMPHOCYTE DEVELOPMENT Immunoglobulin Rearrangement Human B-cell antigen receptor genes include the immunoglobulin (Ig) heavy-chain locus (IGH) at 14q32, the kappa…
Flow Cytometric Analysis in Hematopathology Diagnosis Gerard Lozanski Alejandro A. Gru INTRODUCTION Multicolor flow cytometric analysis (FCA) is routinely used in diagnostic hematopathology. FCA allows for rapid and accurate characterization…
Molecular Biology of T-Cell Lymphoma Anjali Mishra Pierluigi Porcu INTRODUCTION T-cell lymphomas represent a group of lymphoproliferative disorders of T-cell origin. T-cell lymphomas tend to be rare, heterogeneous, and more…
