Biohardening of Micropropagated Plants with PGPR and Endophytic Bacteria Enhances the Protein Content


Sample no.

Crop field/Grounds

Place

1

Paddy field

Manoharabad, Rangareddy district

2

Vegetable field

Anakapally, Vizag district

3

Ground nut field

Nandyal, Kurnool district

4

Sun flower field

Miriyalaguda, Nalgonda district

5

Red gram field

Eluru, East Godavari district

6

Pearl Millet field

Sngareddy, Medak district

7

Sesame field

Aurmoor Nizamabad district

8

Sea coast

Vizag. Vishakhapatnam district

9

Dump site

Hussain Sagar Lake, Hyderabad district



Each bacterium showed varied colony forming units, the actual number of CFU was analyzed for the inoculation considering the volume of loop to be 0.01 ml.

The viable counts ranged in the soil samples were Rhizobium (2.41 × 104 CFU/ml), Pseudomonas (1.42 × 103 CFU/ml), PSB (1.5 × 106 CFU/ml), Azospirillum (1.82 × 105 CFU/ml), Acetobacter (2.0 × 107 CFU/ml).

Conformation of the Bacterial samples was analyzed by performing grams staining, IMVIC tests [9] (Table 6.2).


Table 6.2
Confirmative test for soil bacteria



































































Test
Rhizobium

Pseudomonas

PSB
Acetobacter

Azospirillum

Gram’s staining
−ve
−ve
+ve
−ve
−ve

Methyl red
+ve
−ve
+ve
+ve
+ve

Voges-Proskaeur
−ve
−ve
+ve
−ve
+ve

Indole
−ve
−ve
−ve
−ve
+ve

Citrate
−ve
+ve
+ve
+ve
−ve

Catalase
+ve
+ve
+ve
+ve
+ve

Oxidase
+ve
+ve
+ve
+ve
+ve



6.9.2 Plant Isolation


When there was Single, dual, triple, four, five and the consortium of the bacterial combinations were inoculated to the tissue cultured plants, the root length, shoot length, no of leaves and the leaf primordial of the plantlets were observed and documented [9].

The Mean concentration of macro nutrients is in the order of (highest to lowest): K (1.74) > N (1.43) > P (0.09) was recorded. Table 6.3 shows that the nitrogen content was less in the plants which were treated with the Consortium and slightly lower with a difference of 0.43 % in the plants from the AG Biotech laboratory treated plants along with the consortium, Phosphorous content is less with a difference of 0.16 %, and potassium with 1.03 %.
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Oct 21, 2016 | Posted by in BIOCHEMISTRY | Comments Off on Biohardening of Micropropagated Plants with PGPR and Endophytic Bacteria Enhances the Protein Content

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