Fig. 1.1
Agarose gel of the polymerase chain reaction with amplified TPI gene from Lactobacillus delbrueckii subsp. lactis incorporating template DNA purified using our rapid procedure. Lanes: 1, 100 ng XVII500-bp PCR marker (10,000) 50 bp; 2 and 3, 4 ll of the corresponding PCR product directly using L. delbrueckii
1.2.8 Bioinformatics Analysis
SDSC BIOLOGY WORK BENCH is a tool kit which provides easy access for various bioinformatics tools to analyze the data. CLUSTAL W tool a product of SDSC was used to perform multiple sequence analysis of the TPI protein among different organisms. Phylogenetic study was made using the DENDROGRAM (Fig. 1.2). TEXSHADE and BOXSHADE were used to identify the common conserved regions among the sequences (Figs. 1.3, 1.4).
Fig. 1.2
The dendrogram indicates that query and Lactobacillus delbrueckii fall under common branch which shows that they share maximum similarity where as Homo sapiens falls under a completely different branch which indicates there evolutionary divergence
Fig. 1.3
This figure infers that most of the regions on the protein are conserved among all the organism
Fig. 1.4
This figure infers that most of the regions on the protein are conserved among all the organism
1.3 Results and Discussion
Different Lactobacillus strains will produce bacteriocins but the Lactobacillus delbrueckii strain is mostly used for the production of more amount of Bacteriocin as the TPI gene is only present in the Lactobacillus delbrueckii strain so the Lactobacillus delbrueckii is isolated from raw cow milk and the Lactobacillus delbrueckii strain were identified by several biochemical tests and the confirmatory test was mannitol test which shows no acid and no gas production which confirms the presence of the Lactobacillus delbrueckii and genomic DNA was isolated by proteinase K method and its TPI gene was amplified by using PCR. The Bacteriocin produced by the Lactobacillus delbrueckii is used as preserving agent in dairy and food industries and it used to prepare probitics to treat several diseases caused by bacteria. The Bacteriocin is used in the preparation of sporlac powder which is used to treat diarrhea. The Bacteriocin produced by the Lactobacillus strains is called lacticin which is mainly used in the food preserving and diary industries.
Stay updated, free articles. Join our Telegram channel
Full access? Get Clinical Tree
