Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane

Mar 20, 2017 by in GENERAL SURGERY Comments Off on Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane

(1) BIOTEC, Biophysics, Technische Universität Dresden, Dresden, Germany (2) Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, Germany   Abstract Scanning fluorescence correlation spectroscopy (SFCS) with a scan…

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Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Circumventing the Diffraction Limit of Light

Mar 20, 2017 by in GENERAL SURGERY Comments Off on Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Circumventing the Diffraction Limit of Light

(1) Department of Chemistry, KU Leuven, Leuven, Belgium   Abstract In the last two decades, fluorescent proteins became an indispensable tool to noninvasively label a protein in living cells. The…

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Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells

Mar 20, 2017 by in GENERAL SURGERY Comments Off on Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells

(1) BIOTEC, Biophysics, Technische Universität Dresden, Dresden, Germany (2) Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, Germany   Abstract Fluorescence correlation spectroscopy (FCS) can add dynamic molecular…

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How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra

Mar 20, 2017 by in GENERAL SURGERY Comments Off on How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra

(1) Fluorescence Division, HORIBA Instruments Inc., Edison, NJ, USA   Abstract Contemporary spectrofluorimeters comprise exciting light sources, excitation and emission monochromators, and detectors that without correction yield data not conforming…

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Time-Resolved Fluorescence Anisotropy Imaging

Mar 20, 2017 by in GENERAL SURGERY Comments Off on Time-Resolved Fluorescence Anisotropy Imaging

, James Levitt1 and Pei-Hua Chung1, 2 (1) Department of Physics, King’s College London, London, UK (2) Faculty of Life Sciences, University of Manchester, Manchester, UK   Abstract Fluorescence can be characterized…

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Wide-Field Fluorescence Lifetime Imaging with Multi-anode Detectors

Mar 20, 2017 by in GENERAL SURGERY Comments Off on Wide-Field Fluorescence Lifetime Imaging with Multi-anode Detectors

(1) Multidimensional Microscopy and Cellular Diagnostics, Medical Faculty, Otto-von-Guericke-University, Magdeburg, Germany (2) Special Laboratory Electron- and Laserscanning Microscopy, Leibniz Institute for Neurobiology (LIN), Magdeburg, Germany   Abstract Fluorescence lifetime imaging…

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High-Pressure Fluorescence Applications

Mar 20, 2017 by in GENERAL SURGERY Comments Off on High-Pressure Fluorescence Applications

(1) INSERM U554, Centre de Biochimie Structurale, Montpellier, France (2) Centre de Biochimie Structurale, INSERM U554, Montpellier, France   Abstract Fluorescence is the most widely used technique to study the…

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Upconversion Spectrophotofluorometry

Mar 20, 2017 by in GENERAL SURGERY Comments Off on Upconversion Spectrophotofluorometry

(1) NIDDK, National Institutes of Health, Bethesda, MD, USA (2) NHLBI, National Institutes of Health, Bethesda, MD, USA   Abstract As the other chapters attest, sensitivity of fluorescent molecules to…

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Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family

Mar 20, 2017 by in GENERAL SURGERY Comments Off on Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family

(1) Faculté de Parmacie, Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Illkirch, France   Abstract Environment-sensitive fluorescent membrane probes are attractive tools for investigating the membrane…

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