Solution. 4 × 107 CFU
CALCULATION
0.1 ml = 4 × 107 CFU = 40 million colony-forming units
8. A 5 mL vial of DTP vaccine (diphtheria, tetanus, and pertussis) contains 40 PU (protective units) of pertussis. If the standard schedule of administration is a total of 16 PU of pertussis administered at 2, 4, 6, and 18 months of age, how many milliliters would be needed for each dose?
Solution. 0.5 mL
CALCULATIONS
= 4 PU/dose
9. The concentration of a typhoid vaccine product is 8 units/mL. For adults and children older than 10 years of age, the full dose is two 0.5 mL SC 4 weeks apart. How many individuals can be fully vaccinated with a 10 mL vial?
Solution. 10 individuals
CALCULATIONS
× 1 mL = 8 units / full dose
× 10 mL (vial) = 80 units/vial
80 units × 
= 10 doses
10. Measles virus vaccine live contains not less than 1000 TCID50 per 0.5 mL dose. How many doses can be administered from a 10 mL multidose vial?
Solution. 20 doses
CALCULATIONS
11. Dosage recommendations by CDC and FDA of a hepatitis B vaccine product for both pre- and post-exposure prophylaxis of adults is 20 micrograms by IM injection at 0, 1, and 6 months. How many patients will be completely immunized from a 10 mL multidose vial containing 200 μg?
Solution. 3 patients
CALCULATIONS
20 μg × 3 doses = 60 μg (per patient)
60 μg (patient) × 
= 3 patients
12. A poliovirus vaccine inactivated product contains 40, 8, and 32 D-antigen units per 0.5 mL dose of poliovirus types 1, 2, and 3, respectively. How many antigen units of each poliovirus type would a child receive from a complete immunization that includes 3 doses?
Solution. 120, 24, and 96 antigen units of poliovirus types 1, 2, and 3, respectively.
CALCULATIONS
3 doses = 120 units of Type 1
3 doses = 24 units of Type 2
3 doses = 96 units of Type 3
13. A traveler, visiting a foreign area of enzootic rabies, was prescribed to receive vaccination for pre-exposure prophylaxis. Primary vaccination schedule was 1 mL IM or 0.1 mL ID of human diploid cell rabies vaccine on days 0, 7, and 21. Imovax® Rabies Vaccine is available in products for intramuscular (IM) injection, containing not less than 2.5 IU/mL, and for intradermal (ID) injection, containing 0.25 IU/0.1 mL. If this patient receives all three doses as scheduled, how many international units (IU) he will receive from either IM or ID type of vaccination schedule?
Solution. 7.5 international units by IM and 0.75 IU by ID
CALCULATIONS
IM vaccination: 
ID vaccination: 
14. A test of hypersensitivity for Yellow Fever vaccine requires injection of 0.02 mL intradermally. If a yellow fever vaccine product contains 0.7 plaque-forming units (PFU) per 0.5 mL, how many PFU will be injected?
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