and Epigenomic Aspects of Gene Expression

Figure 3-8 Schematic representation of chromatin and three major epigenetic mechanisms: DNA methylation at CpG dinucleotides, associated with gene repression; various modifications (indicated by different colors) on histone tails, associated with either gene expression or repression; and various histone variants that mark specific regions of the genome, associated with specific functions required for chromosome stability or genome integrity. Not to scale.

Increasing evidence points to a role for epigenetic changes in human disease in response to environmental or lifestyle influences. The dynamic and reversible nature of epigenetic changes permits a level of adaptability or plasticity that greatly exceeds the capacity of DNA sequence alone and thus is relevant both to the origins and potential treatment of disease. A number of large-scale epigenomics projects (akin to the original Human Genome Project) have been initiated to catalogue DNA methylation sites genome-wide (the so-called methylome), to evaluate CpG landscapes across the genome, to discover new histone variants and modification patterns in various tissues, and to document positioning of nucleosomes around the genome in different cell types, and in samples from both asymptomatic individuals and those with cancer or other diseases. These analyses are part of a broad effort (called the ENCODE Project, for Encyclopedia of DNA Elements) to explore epigenetic patterns in chromatin genome-wide in order to better understand control of gene expression in different tissues or disease states.

DNA Methylation


Figure 3-9 The modified DNA bases, 5-methylcytosine and 5-hydroxymethylcytosine. Compare to the structure of cytosine in Figure 2-2. The added methyl and hydroxymethyl groups are boxed in purple. The atoms in the pyrimidine rings are numbered 1 to 6 to indicate the 5-carbon.

Extensive demethylation occurs during germ cell development and in the early stages of embryonic development, consistent with the need to “re-set” the chromatin environment and restore totipotency or pluripotency of the zygote and of various stem cell populations. Although the details are still incompletely understood, these reprogramming steps appear to involve the enzymatic conversion of 5-mC to 5-hydroxymethylcytosine (5-hmC; see Fig. 3-9), as a likely intermediate in the demethylation of DNA. Overall, 5-mC levels are stable across adult tissues (approximately 5% of all cytosines), whereas 5-hmC levels are much lower and much more variable (0.1% to 1% of all cytosines). Interestingly, although 5-hmC is widespread in the genome, its highest levels are found in known regulatory regions, suggesting a possible role in the regulation of specific promoters and enhancers.

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Nov 27, 2016 | Posted by in GENERAL & FAMILY MEDICINE | Comments Off on and Epigenomic Aspects of Gene Expression

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